Showing posts with label identification. Show all posts
Showing posts with label identification. Show all posts

Tuesday, April 9, 2024

Extraction of Insect DNA From The Air!

    This article details how we have a high estimate of the total number of species on this planet. It discusses how many species we have discovered in comparison to our early highest estimates, and provides a potential solution. Our current method of identifying new species around the world is incredibly slow, only moving forward by people actively going in the field, collecting, and describing species. Additionally, rates of species discovery grow increasingly slower as the species decrease in size. However, this new form of technology would allow the capture of DNA from within the air. It costs less, and is faster than traditional sampling methods, and, additionally, can ID many species simultaneously. Airborne samples are collected using something called a liquid cyclone contraption. This pulls air into a liquid filled tube, trapping DNA fragments. This then allows for DNA extraction, amplification, and sequencing. This method is particularly efficient with insect species, which is significant because insects are the most diverse group of organisms on the planet. This article stresses how species identification is very important for conservation efforts. 

    I find this article to be incredibly interesting. As someone who actively studies insects, I feel as though this new method is far more efficient when it comes to insect identification and the identification of new insect species. it would also in theory utilize less resources, as now individuals studying the insects won't have to spend as much time in the field, therefore reducing the need for long term camping, and other resource use in the field. I can foresee some potential problems, however this is a fantastic starting point, and feels almost like the work of science fiction. I cannot wait to see where this research goes.

(Pictured is an example of a liquid cyclone separator)

Saturday, February 19, 2022

Scientists Discover Genes that Make Your Fingerprints

 

    According to an article from U.S. News, scientists have discovered that human fingerprint patterns are linked with genes responsible for limb growth during fetal development. After looking at DNA from over 23,000 individuals, scientists found 43 genomic regions that are associated with fingerprint patterns. The region with the most influence is known as EVI1, and it plays a critical role in embryotic limb development. To test their findings, they restricted the expression of EVI1 in mice, and this resulted in the mice having abnormal patterns on their digits. 

   The article also talked about how previous studies had suggested that EVI1 is linked to leukemia risks, and that it was observed that people who were more susceptible to leukemia had more whorl patterns on their fingers. The researchers all agree that more investigation is needed to truly understand the relationship between fingerprints, genes, and diseases. 

    I found this study very fascinating because it had never occurred to me that fingerprints could be related to genetics. I also did not know that some fingerprint patterns are being associated with diseases like leukemia. I believe this will expand the use of the fingerprint long past simply identifying a person but being able to determine a better profile of a person based on their fingerprint patters.

    Related Article

Fingerprints and Science – Fingerprinting in the Modern World (rutgers.edu)


Thursday, April 6, 2017

Is it a Boy or a Girl?- Baby Sea Turtles


        New research has come about that is able to help determine the gender of baby sea turtles. In most species the gender is determined if there is and X and Y chromosome present or just two X chromosomes. This however is not the case with sea turtle since they do not have sex chromosomes. Instead the sea turtles gender is determined as they develop in their incubation state. The biggest contributing factor that influences the gender is the environment the turtle is living in. It has been found that warmer weather and sands leads to females and colder temperature and sand produces males. It is hard to conform this due to the fact that the sea turtles sexual anatomy isn't distinguishable until they are full mature.
       Scientists from Florida Atlantic University have recently begun using immunohistochemical (IHC). This practice allows researchers to identify the sex without harming the species, take into account sex ratios, and acknowledge the relationship between the environment and the sex.
       Research as also found that the RNA binding protein, CIRPB could be responsible determining the sex of the turtles. CIRPB is temperature responsive which supports the idea that certain temperatures produce a male while others produce a female.

Wednesday, December 3, 2014

Scientists Confirm the Skeleton of Richard III by DNA Analysis


A skeleton found underneath a parking lot in Leicester, England believed to be the remains of King Richard III was recently confirmed by DNA analysis of the remains as well as tracing the genetic evidence over 20 generations. This is the oldest DNA identification of any known person. The head geneticist of the University of Leicester stated, "Even with our most conservative statistical analysis, we're 99.999% sure." Along with the biological data, the historical data and recorded place, time, and cause of death also prove to confirm this identification. Retrieving 500 year old DNA was not an easy task. The researchers had to go through millions of ribbons of splintered DNA fragments. By matching mitochondrial DNA and following the passing down of the Y chromosome, it was confirmed that the remains were indeed of King Richard III. (And also stirred up proof of infidelity while they were at it.)

Original Article: http://www.popularmechanics.com/science/health/genetics/scientists-confirm-remains-king-richard-iii-17487010

Related Article: http://news.nationalgeographic.com/news/2014/12/141202-richard-iii-genes-shakespeare-science/

Tuesday, December 2, 2014

Royal Bones Found in Parking Lot

Two years ago an old skeleton was found under a parking lot in Leicester England. The archaeologists who found the skeleton claimed they found the bones of Richard III, a 15th century monarch. This claim was based on the fact that the skeleton was male, the right age, had battle injuries, and scoliosis. The scientists couldn't be entirely sure it was him so of course DNA evidence was needed.
Image from http://www.thetimes.co.uk/tto/multimedia/archive/00380/RichardIII_380709a.jpg
Today, December 2nd, it was announced that the DNA analysis of the skeleton was completed. It was reported in Nature Communications. The DNA analysis proved that they bones did belong to the former king. The scientists were able to trace the genetic evidence through more than 20 generations of the dead King's family tree to his living descendants. This means that the oldest DNA identification case of known individual was completed.  Turi King, the lead geneticist who lead the analysis stated that they were even 99.999% sure that the remains belong to Richard III.
What the team did was scrape together a DNA profile from a very old skeleton. Since the DNA was so old and damaged, scientists had to look through millions of ribbons of splintered DNA strings. Also to make sure the analysis wasn't contaminated, scientists did the sequencing in multiple clean rooms at multiple locations. Then the profile was compared to Richard III's living relatives. That was still difficult though because an ancestor from 20 generations back would only contribute .000001 of an individual's DNA. What does go almost unchanged through generations is mitochondrial DNA and the DNA on the Y chromosome. Mitochondrial DNA only comes from the mother allowing scientists to look at an all female line through the family tree. It was discovered that the descendants had the exact same mitochondrial DNA except for 1 single mutation. Richard did not have a pure male-line descendants making the tracking of the Y chromosome difficult. The team had more than enough information though.
I thought this was extremely interesting and relevant to class because we were looking at case studies similar to this with mummies and learning about how difficult it is to extract DNA from the dead and how there is a great risk of contamination. It is amazing what can be done with information on someone's DNA.

Article: http://www.popularmechanics.com/science/health/genetics/scientists-confirm-remains-king-richard-iii-17487010