This study was to analyze the variant of the conventional protein Kinase C-γ (PKCγ) rs1331262028 associated with ovarian cancer and to determine its impact on PKCγ’s protein interactions. The impact of this variation on PKCγ interactions was determined by genotyping PCR (cohort size:100), protein–protein docking and molecular dynamic simulation. To conduct these tests; first: samples from patients with ovarian cancer were collected and processed. Second: genomic DNA was extracted for genotype analysis. The third step used In-situ mutagenesis, followed by statistical examination and Molecular Docking of PKCγ with Connexin43. Finally, the interaction dynamics were analyzed, as well as the Pathway Construction for PKCγ’ and Connexin43 Interaction. The outcome of these tests indicates the positive association of variant rs1331262028 with ovarian cancer and its clinicopathological features. Molecular dynamics simulation depicts the potential influence of variation on PKCγ molecular signaling. This study has begun to create a foundation for assessing this variant as a possible prognostic marker for ovarian cancer. Further research can build upon these results.
This study could assist in finding better indicators for cancer causing genes so that cancer, in individuals, can be treated earlier than they would have been previously. Though the presence of this variation in PKCy may indicate the possibility of the individual becoming symptomatic, it is not a guarantee. This could possibly lead to a false positive in diagnosis. It is still a step closer to understanding carcinogens, but it is not absolute in its diagnosis capabilities.
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