It is always vital to know what is fairly recent in the world of a geneticist. Learning which method of sequencing will help the process of depicting snips. The research article, Interaction between host microRNAs and the gut microbiota in colorectal cancer. ASM, 3(3): 1-13, has found microRNA plays apart as a "host-microbiome" in colorectal cancer.
“It is important to note that our study uses 16S rRNA gene sequencing to characterize microbiome taxonomic composition and computationally predicted pathway composition using PICRUSt v1.0.0 (71). Although this method is widely used, metagenomic shotgun sequencing can be more accurate and informative in understanding the functional makeup of a microbial community. Similarly, to impute miRNA functional profiles, we used an in silico prediction method, miRPath (71, 72). While both of these methods have been rigorously tested and validated with experimental data, the results remain predictions and may not represent the real biological system (71, 72). Another limitation of our approach is that it identifies correlations and not causal relationships. Nevertheless, this approach allows us to generate a microbiome- and miRNA transcriptome-wide characterization of potential interactions, which shed light on potential new mechanisms of host-microbiome interactions.” (Yuan et al 2018). This study is using specific techniques to receive their answers. It then shows these techniques do have their benefits with certain aspects but has its drawbacks
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