This fertilization technique involved placing 19 fertile embryos into a host female dog. The mother gave birth to 7 healthy puppies. The embryos don't have a 100% success rate but this process has been tried for the last 40 years and was finally successful. This was done by fertilizing the mature egg with the sperm cells outside of the host, and then placing them in the host mother.
There were a few challenges in this process though. For example, collecting the right maturity of egg cells was a problem. The reproductive cycle of a dog is different from other mammals, so finding mature eggs were a little more difficult. Another challenge was simulating the environment in which the egg is fertilized naturally. This is difficult to do in the lab. The final challenge was freezing the embryo, so it could be placed in the oviducts at the proper time.
These types of techniques can be used to preserve breeds of rare show or work dogs. This also could help bring back important genes back into the gene pool. Eventually, these and gene editing techniques could be used to ridding heritable diseases in dogs and possibly humans. This study opens the door to many future discoveries.
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