Friday, September 26, 2014

Potential Cure for HIV
According to the World Health Organization (WHO), approximately 0.8% of adults between the ages of 15-49 years old are living with human immunodeficiency virus (HIV) worldwide. Despite the global prevalence of the virus, there has not yet been a functional cure. However, recently an HIV-positive patient with leukemia was cured of the virus. The patient underwent total body irradiation (TBI) and a bone-marrow transplant. The bone-marrow donor had a mutation which prevents the function of the CCR5 gene. The CCR5 gene codes for a protein which allows HIV to enter human cells. The mutation the donor possessed, however, protects cells against HIV infection.

Experts believe three factors may have contributed to the cure in the Berlin patient. The first factor which may have contributed to the patient’s cure is that the removal of blood and immune cells  which occurred after irradiation killed many of the viral reservoir cells. The second contribution may have been that the CCR5 deletion mutation from the donor cells protected those cells and their progeny from HIV infection. The third contribution to the patient’s cure may have been a graft versus host reaction which resulted in the transplanted cells and their progeny attacking and eliminating the remaining HIV-positive cells.
In order to further research this potential cure for HIV, a study was conducted involving six rhesus macaques infected with simian immunodeficiency virus (SIV). The researchers harvested hematopoietic stem cells from three of the six of the macaques prior to infection with SIV and treated the macaques with antiretroviral therapy (ART). They treated the three macaques from which they collected hematopoietic stem cells with high doses of radiation. The radiation killed 94%-99% of the monkeys’ CD4-T cells. The three monkeys then received their own virus-free hematopoietic stem cells. The viral load rebounded rapidly in the three control group monkeys and in two of the monkeys who received the transplant. Due to the failure of the treatment, the researchers suggest that “the use of the CCR5 mutant donor and/or the presence of graft versus host disease played a significant role” in the cure of the Berlin patient.
Overall, this potential treatment for HIV seems to have promise and should definitely be more extensively researched. This treatment involving ART, irradiation, and a hematopoietic stem cell transplant is very complex, and there are many aspects of it which should be thoroughly examined. I found this article extremely interesting because the graft versus host disease in the Berlin patient seemed to have been a vital factor in how the patient was cured of HIV. Due to the complexity of the Berlin patient’s case, the results will be difficult to replicate; however, replication of the Berlin patient’s results has profound implications in the search for a cure for HIV.



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