tag:blogger.com,1999:blog-3360356351594869925.post3776541877201474981..comments2024-03-28T15:16:41.477-04:00Comments on Punnett's Square: CRISPR CAS9 ComplicationsG. F. Barbatohttp://www.blogger.com/profile/09416043925683981826noreply@blogger.comBlogger2125tag:blogger.com,1999:blog-3360356351594869925.post-13047753020633020472018-12-12T18:05:32.316-05:002018-12-12T18:05:32.316-05:00This is something I did not know about Crispr. I f...This is something I did not know about Crispr. I figured that it just silenced the gene sequence and did not take into account the fact that we have response mechanisms for fixing mutations in our genomic sequences. Perhaps this is the reason why we can't use Crispr to silence entire chromosomes because there wouldn't be any progress. It will be interesting to see if they can develop something to block these response mechanisms such as p53. Nick Rosahttps://www.blogger.com/profile/12274050831229767330noreply@blogger.comtag:blogger.com,1999:blog-3360356351594869925.post-48073639167677970212018-12-09T19:04:04.295-05:002018-12-09T19:04:04.295-05:00Although NHEJ is the primary method of DNA repair ...Although NHEJ is the primary method of DNA repair for most mammalian species, it has been found that HDR is a more dominant method of DNA repair in budding yeasts in a controlled lab scenario. If the scientific community were to somehow identify the biochemical process on why this occurs to only yeast. We may be able to use that information to enhance the CRISPR technology. Anthony Brigatihttps://www.blogger.com/profile/13939205922210836590noreply@blogger.com